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KMID : 1094720140190020327
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Biotechnology and Bioprocess Engineering
2014 Volume.19 No. 2 p.327 ~ p.331
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Improvement of gamma-amino butyric acid production by an overexpression of glutamate decarboxylase from Pyrococcus horikoshii in Escherichia coli
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Vo Tam Dinh Le
Pham Van Dung
Ko Ji-Seun
Lee Seung-Hwan
Park Si-Jae
Hong Soon-Ho
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Abstract
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In this study, the effect of glutamate decarboxylase from Pyrococcus horikoshii on gamma-aminobutyric acid (GABA) production was investigated in Escherichia coli for the first time. E. coli with overexpressed P. horikoshii glutamate decarboxylase was cultured at various pH levels and temperatures to determine the optimum conditions for GABA production. The highest final GABA concentration, 5.07 g/L, was obtained from 10 g/L of monosodium glutamate (MSG) with a GABA yield of 83% at 30°C and pH 3.5. When P. horikoshii glutamate decarboxylase was introduced into a GABA aminotransferase knock-out E. coli XBT strains, 5.69 g/L of GABA was produced with a GABA yield of 93%.
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KEYWORD
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Escherichia coli, gamma-aminobutyric acid, glutamate decarboxylase, Pyrococcus horikoshii
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